The Hidden Cost of Antihistamines: How Blocking Histamine Silences Your Most Important Neural Network — section

ACh is synthesized from two precursors: acetyl-CoA (a mitochondrial metabolite) and choline (a dietary nutrient). The enzyme choline acetyltransferase (ChAT) combines these to produce acetylcholine, which is then packaged into vesicles and released at nerve terminals.